{"term":{"id":"08347","title":"H(CCO)NH","longtitle":"IUPAC Gold Book - H(CCO)NH","doi":"10.1351\/goldbook.08347","code":"08347","status":"current","definitions":[{"id":1,"text":"Three-dimensional nuclear magnetic resonance spectroscopy for the assignment of protein structure with one nitrogen, and two hydrogen dimensions.","notes":{"1":"Magnetization is transferred from the side-chain hydrogen nuclei to their attached \\(\\ce{^{13}C}\\) nuclei. Then isotropic \\(\\ce{^{13}C}\\) mixing is used to transfer magnetization between the carbon nuclei. From here, magnetization is transferred to the carbonyl carbon, on to the amide nitrogen and finally the amide hydrogen for detection.","2":"Requires \\(\\ce{^{15}N}\\) and \\(\\ce{^{13}C}\\) labelling.","3":"Useful spectrum for obtaining hydrogen side-chain assignments."},"links":[{"term":"Three-dimensional nuclear magnetic resonance spectroscopy","url":"https:\/\/goldbook.iupac.org\/terms\/view\/08432"},{"term":"spectrum","url":"https:\/\/goldbook.iupac.org\/terms\/view\/08384"}],"sources":["PAC, 2021, 93, 647. 'Glossary of methods and terms used in analytical spectroscopy (IUPAC Recommendations 2019)' on page 689 (https:\/\/doi.org\/10.1515\/pac-2019-0203)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/08347\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/08347\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/08347\/plain"},"citation":"Citation: 'H(CCO)NH' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.08347","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-07-27T05:00:25+00:00"}}