Title: indirect immunofluorescence
Long Title: IUPAC Gold Book - indirect immunofluorescence
DOI: 10.1351/goldbook.13328
Status: current

Definition
Method of visualizing an antigen in which an unlabeled antibody against the antigen is reacted with it first, and then is detected by staining with a second fluorescently tagged immunoglobulin G (IgG) directed against the first antibody.

Related Term
- immunoglobulin G (IgG): https://goldbook.iupac.org/terms/view/13292

Source
- PAC, 2012, 84, 1113. 'IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)' on page 1193 (https://doi.org/10.1351/PAC-REC-11-06-03)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/13328/html
- json: https://goldbook.iupac.org/terms/view/13328/json
- xml: https://goldbook.iupac.org/terms/view/13328/xml

Citation: Citation: 'indirect immunofluorescence' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.13328
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Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2026-07-24T10:56:44+00:00