{"term":{"id":"14111","title":"binding assay","longtitle":"IUPAC Gold Book - binding assay","doi":"10.1351\/goldbook.14111","code":"14111","status":"current","definitions":[{"id":1,"text":"Assay in which the specific physical association or interaction between two molecules (e.g., ligand–receptor, antibody–antigen, protein–protein, ligand–transport protein) is measured.","notes":{"1":"The assay can be homogeneous or heterogeneous, competitive or noncompetitive, and may be run at equilibrium or in kinetic mode. Appropriate assay controls and\/or standard reagents are often needed to determine the specific binding in contrast to nonspecific adsorption processes."},"sources":["PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1133 (https:\/\/doi.org\/10.1351\/PAC-REC-09-05-03)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/14111\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/14111\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/14111\/plain"},"citation":"Citation: 'binding assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.14111","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-06-27T15:22:43+00:00"}}