{"term":{"id":"14133","title":"flow cytometry assay","longtitle":"IUPAC Gold Book - flow cytometry assay","doi":"10.1351\/goldbook.14133","code":"14133","status":"current","definitions":[{"id":1,"text":"Cell-based assay in which the response is dependent on the detection of the phenotype of cells as determined through the light-scattering properties of each cell, and, typically, the fluorescence intensity of a relevant probe.","notes":{"1":"Flow cytometry has been adapted to fluorescently labeled bead-based assays.","2":"In fluorescence-activated cell sorting, single cells are rapidly passed through a channel where they are optically analyzed and separated based on the specific light-scattering and fluorescent characteristics of each individual cell."},"links":[{"term":"Cell-based assay","url":"https:\/\/goldbook.iupac.org\/terms\/view\/14113"}],"sources":["PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1140 (https:\/\/doi.org\/10.1351\/PAC-REC-09-05-03)"]}],"altoutputs":{"html":"https:\/\/goldbook.iupac.org\/terms\/view\/14133\/html","xml":"https:\/\/goldbook.iupac.org\/terms\/view\/14133\/xml","plain":"https:\/\/goldbook.iupac.org\/terms\/view\/14133\/plain"},"citation":"Citation: 'flow cytometry assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351\/goldbook.14133","license":"The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https:\/\/creativecommons.org\/licenses\/by-sa\/4.0\/) for individual terms.","collection":"If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .","disclaimer":"The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.","accessed":"2026-05-10T17:07:19+00:00"}}