Title: kinetic assay
Long Title: IUPAC Gold Book - kinetic assay
DOI: 10.1351/goldbook.14152
Status: current

Definition
Assay in which the time dependence of the signal intensity is measured. Signal values are typically acquired at several time intervals in order to calculate kinetic parameters.

Note
Kinetic assays are generally designed such that the change in signal with time is linear throughout the experiment.

Related Terms
- end-point assay: https://goldbook.iupac.org/terms/view/14128
- equilibrium assay: https://goldbook.iupac.org/terms/view/14130

Source
- PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1145 (https://doi.org/10.1351/PAC-REC-09-05-03)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/14152/html
- json: https://goldbook.iupac.org/terms/view/14152/json
- xml: https://goldbook.iupac.org/terms/view/14152/xml

Citation: Citation: 'kinetic assay' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14152
License: The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms.
Collection: If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org .
Disclaimer: The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using.
Accessed: 2026-06-14T11:01:46+00:00