14182 IUPAC Gold Book - positive assay control 10.1351/goldbook.14182 14182 current synonym: high control 1 Experimental conditions designed to produce the signal reflective of maximum biological effect in an assay. For a binding or an activation mechanism, it is typically the signal measured in the presence of a test compound. For an inhibitory or antagonist mechanism, it is the minimum signal, obtained in the absence of the reference inhibitor. PAC, 2011, 83, 1129. 'Glossary of terms used in biomolecular screening (IUPAC Recommendations 2011)' on page 1151 (https://doi.org/10.1351/PAC-REC-09-05-03) https://goldbook.iupac.org/terms/view/14182/html https://goldbook.iupac.org/terms/view/14182/json https://goldbook.iupac.org/terms/view/14182/plain Citation: 'positive assay control' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.14182 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-06-27T15:20:33+00:00