16258 IUPAC Gold Book - total mobile-phase volume in column 10.1351/goldbook.16258 16258 current 1 Sum of the interparticle volume of the column and intraparticle volume of the column: \(V_{\rm{t}} = V_{0} + V_{\rm{i}}\) In the definition of \(V_{\rm{t}}\) the extra-column volume of the system (\(V_{\rm{ext}}\)) is neglected. If it is not negligible, it must also be added: \[V_{\rm{t}} = V_{0} + V_{\rm{i}} + V_{\rm{ext}}\] intraparticle volume of the column https://goldbook.iupac.org/terms/view/11549 PAC, 2017, 90, 181. 'Terminology of separation methods (IUPAC Recommendations 2017)' on page 211 (https://doi.org/10.1515/pac-2017-0111) https://goldbook.iupac.org/terms/view/16258/html https://goldbook.iupac.org/terms/view/16258/json https://goldbook.iupac.org/terms/view/16258/plain Citation: 'total mobile-phase volume in column' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.16258 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-07-27T01:01:47+00:00