02249 IUPAC Gold Book - excitation-emission spectrum 10.1351/goldbook.E02249 E02249 current initialism: EES 1 The three-dimensional spectrum generated by scanning the emission spectrum at incremental steps of excitation wavelength (\(x\) axis = emission wavelength, \(y\) axis = excitation wavelength, \(z\) axis = emission flux) is called a (fluorescence, phosphorescence) excitation-emission spectrum (or EES). The spectra are particularly useful for investigating samples containing more than one emitting species. Corrected EES are obtained if (a) the emission is corrected for instrumental response with wavelength, and (b) the exciting radiation flux in photons \(\rm{s}^{-1}\) is held constant for all excitation wavelengths. emission spectrum https://goldbook.iupac.org/terms/view/E02060 excitation https://goldbook.iupac.org/terms/view/E02248 fluorescence https://goldbook.iupac.org/terms/view/F02453 phosphorescence https://goldbook.iupac.org/terms/view/P04569 wavelength https://goldbook.iupac.org/terms/view/W06659 PAC, 1984, 56, 231. 'Nomenclature, symbols, units and their usage in spectrochemical analysis-Part VI: molecular luminescence spectroscopy' on page 242 (https://doi.org/10.1351/pac198456020231) https://goldbook.iupac.org/terms/view/E02249/html https://goldbook.iupac.org/terms/view/E02249/json https://goldbook.iupac.org/terms/view/E02249/plain Citation: 'excitation-emission spectrum' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.E02249 The IUPAC Gold Book is licensed under Creative Commons Attribution-ShareAlike CC BY-SA 4.0 International (https://creativecommons.org/licenses/by-sa/4.0/) for individual terms. If you are interested in licensing the Gold Book for commercial use, please contact the IUPAC Executive Director at executivedirector@iupac.org . The International Union of Pure and Applied Chemistry (IUPAC) is continuously reviewing and, where needed, updating terms in the Compendium of Chemical Terminology (the IUPAC Gold Book). Users of these terms are encouraged to include the version of a term with its use and to check regularly for updates to term definitions that you are using. 2026-06-28T13:52:20+00:00