Title: exclusion chromatography
Long Title: IUPAC Gold Book - exclusion chromatography
DOI: 10.1351/goldbook.E02261
Status: current

Definition
Chromatography in which separation is based mainly on exclusion effects, such as differences in molecular size and/or shape or in charge. The term size-exclusion chromatography may also be used when separation is based on molecular size. The terms gel filtration and gel-permeation chromatography (GPC) were used earlier to describe this process when the stationary phase is a swollen gel. The term ion-exclusion chromatography is specifically used for the separation of ions in an aqueous phase.

Related Terms
- chromatography: https://goldbook.iupac.org/terms/view/C01075
- filtration: https://goldbook.iupac.org/terms/view/F02388
- gel: https://goldbook.iupac.org/terms/view/G02600
- permeation chromatography: https://goldbook.iupac.org/terms/view/P04505

Source
- PAC, 1993, 65, 819. 'Nomenclature for chromatography (IUPAC Recommendations 1993)' on page 826 (https://doi.org/10.1351/pac199365040819)

Other Outputs
- html: https://goldbook.iupac.org/terms/view/E02261/html
- json: https://goldbook.iupac.org/terms/view/E02261/json
- xml: https://goldbook.iupac.org/terms/view/E02261/xml

Citation: Citation: 'exclusion chromatography' in IUPAC Compendium of Chemical Terminology, 5th ed. International Union of Pure and Applied Chemistry; 2025. Online version 5.0.0, 2025. 10.1351/goldbook.E02261
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Accessed: 2026-07-23T23:47:01+00:00