indirect immunofluorescence

https://doi.org/10.1351/goldbook.13328
Method of visualizing an antigen in which an unlabeled antibody against the antigen is reacted with it first, and then is detected by staining with a second fluorescently tagged immunoglobulin G (IgG) directed against the first antibody.
Source:
PAC, 2012, 84, 1113. (IUPAC glossary of terms used in immunotoxicology (IUPAC Recommendations 2012)) on page 1193 [Terms] [Paper]