https://doi.org/10.1351/goldbook.14111
Assay in which the specific physical association or interaction between two molecules (e.g., ligand–receptor, antibody–antigen, protein–protein, ligand–transport protein) is measured.
Note: The assay can be homogeneous or heterogeneous, competitive or noncompetitive, and may be run at equilibrium or in kinetic mode. Appropriate assay controls and/or standard reagents are often needed to determine the specific binding in contrast to nonspecific adsorption processes.